selection cassette Search Results


cassettes pre-loaded with dehydrated selective media for specific pathogen detection  (bioMerieux gmbh)
90
bioMerieux gmbh cassettes pre-loaded with dehydrated selective media for specific pathogen detection
Cassettes Pre Loaded With Dehydrated Selective Media For Specific Pathogen Detection, supplied by bioMerieux gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/pm37177723-46-24-29?v=bioMerieux+gmbh
Average 90 stars, based on 1 article reviews
cassettes pre-loaded with dehydrated selective media for specific pathogen detection - by Bioz Stars, 2026-07
90/100 stars
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selectable markers cassettes  (GenScript corporation)
90
GenScript corporation selectable markers cassettes
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Selectable Markers Cassettes, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/pmc09232646-112-5-16?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
selectable markers cassettes - by Bioz Stars, 2026-07
90/100 stars
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cassettes pre-loaded with dehydrated selective media  (bioMerieux gmbh)
90
bioMerieux gmbh cassettes pre-loaded with dehydrated selective media
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Cassettes Pre Loaded With Dehydrated Selective Media, supplied by bioMerieux gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/pmc10181535-84-16-29?v=bioMerieux+gmbh
Average 90 stars, based on 1 article reviews
cassettes pre-loaded with dehydrated selective media - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hdhfr selection cassette  (GenScript corporation)
90
GenScript corporation hdhfr selection cassette
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Hdhfr Selection Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/10__1021_slash_acs__analchem__0c00547-36-1-30?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
hdhfr selection cassette - by Bioz Stars, 2026-07
90/100 stars
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ampicillin selection cassette  (Promega)
90
Promega ampicillin selection cassette
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Ampicillin Selection Cassette, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/10__1074_slash_jbc__m116__755132-305-30-27?v=Promega
Average 90 stars, based on 1 article reviews
ampicillin selection cassette - by Bioz Stars, 2026-07
90/100 stars
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dna fragments corresponding to the screening cassette and to the selection cassette  (GenScript corporation)
90
GenScript corporation dna fragments corresponding to the screening cassette and to the selection cassette
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Dna Fragments Corresponding To The Screening Cassette And To The Selection Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/pmc07884431-295-10-15?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
dna fragments corresponding to the screening cassette and to the selection cassette - by Bioz Stars, 2026-07
90/100 stars
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in vitro selection cassettes (pgk promoter driven turbogfp-t2a-puromycin resistance)  (GenScript corporation)
90
GenScript corporation in vitro selection cassettes (pgk promoter driven turbogfp-t2a-puromycin resistance)
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
In Vitro Selection Cassettes (Pgk Promoter Driven Turbogfp T2a Puromycin Resistance), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/pmc10468692-109-2-19?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
in vitro selection cassettes (pgk promoter driven turbogfp-t2a-puromycin resistance) - by Bioz Stars, 2026-07
90/100 stars
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als r selection cassette  (KU Leuven)
90
KU Leuven als r selection cassette
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Als R Selection Cassette, supplied by KU Leuven, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/pmc02230553-278-37-28?v=KU+Leuven
Average 90 stars, based on 1 article reviews
als r selection cassette - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
selected marker amds expression cassette  (GenScript corporation)
90
GenScript corporation selected marker amds expression cassette
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Selected Marker Amds Expression Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/us11913053-192-14-20?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
selected marker amds expression cassette - by Bioz Stars, 2026-07
90/100 stars
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1,580 cassette containing the three selected grnas  (Synbio Technologies LLC)
90
Synbio Technologies LLC 1,580 cassette containing the three selected grnas
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
1,580 Cassette Containing The Three Selected Grnas, supplied by Synbio Technologies LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/ppr0514499-186-8-12?v=Synbio+Technologies+LLC
Average 90 stars, based on 1 article reviews
1,580 cassette containing the three selected grnas - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
hdhf selection cassette  (GenScript corporation)
90
GenScript corporation hdhf selection cassette
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Hdhf Selection Cassette, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/pmc07203758-6-1-30?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
hdhf selection cassette - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
alsr selection cassette  (KU Leuven)
90
KU Leuven alsr selection cassette
a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating <t>selectable</t> markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.
Alsr Selection Cassette, supplied by KU Leuven, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selection+cassette/pm17921342-257-37-28?v=KU+Leuven
Average 90 stars, based on 1 article reviews
alsr selection cassette - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating selectable markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome

doi: 10.1038/s41467-022-31305-4

Figure Lengend Snippet: a Schematic representation of the circular PGNC construct (PGNC circ ). b Iterative assembly of the PGNC via homologous recombination and using alternating selectable markers. c Confirmation of the PGNC assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were obtained across at least two repetitions and during sub-assembly d Production of three linear PGNC variants using the telomerator (PGNC lin1 , PGNC lin2 and PGNC lin3 ). Yellow boxes define the position of the centromeric-plasmid backbone. Red boxes denote positions of synthetic telomeric repeat sequences added by the telomerator process. e Microplate growth kinetics of the four PGNC variants (PGNC circ , PGNC lin1 , PGNC lin2 and PGNC lin3 ) compared to the BY4742 (WT) strain. Data are presented as mean values +/− SD, based upon values obtained from three independent biological replicates. Source data are provided as a Source Data file.

Article Snippet: All 21 chunks and the selectable markers cassettes were synthesized and cloned into a plasmid vector (GenScript).

Techniques: Construct, Homologous Recombination, Plasmid Preparation